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Anti-TNF alpha antibody [P/T2] (ab9579)

Key features and details

  • Mouse monoclonal [P/T2] to TNF alpha
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-TNF alpha antibody [P/T2]
    See all TNF alpha primary antibodies

  • Description

    Mouse monoclonal [P/T2] to TNF alpha

  • Host species

    Mouse

  • Specificity

    The antibody labels the cytoplasm of TNF-alpha secreting cells including macrophages.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human TNF alpha aa 115-130.
    Database link: P01375

  • Positive control

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [P/T2] (ab9579)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [P/T2] (ab9579)
    The antibody ab9579 labels the cytoplasm of TNF-a secreting cells including histiocytes/macrophages.
  • Flow Cytometry - Anti-TNF alpha antibody [P/T2] (ab9579)
    Flow Cytometry - Anti-TNF alpha antibody [P/T2] (ab9579)
    Overlay histogram showing THP1 cells stained with ab9579 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9579, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [P/T2] (ab9579)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [P/T2] (ab9579)
    Immunohistochemisty of Human adenocarcinoma uterum (with positive reactive macrophages).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [P/T2] (ab9579)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [P/T2] (ab9579)
    Immunochistochemistry of Human mandibular cyst (granulomatous inflammation with positive macrophages).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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