Anti-Lamin A + Lamin C (phospho S392) antibody (ab58528)
Key features and details
- Rabbit polyclonal to Lamin A + Lamin C (phospho S392)
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Lamin A + Lamin C (phospho S392) antibody
See all Lamin A + Lamin C primary antibodies -
Description
Rabbit polyclonal to Lamin A + Lamin C (phospho S392) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of Lamin A + C only when phosphorylated at serine 392. -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Lamin A + Lamin C.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lamin A + Lamin C (phospho S392) antibody (ab58528)
Western blot analysis of HeLa cells labeling Lamin A + C (phospho S392) with ab58528. The lane on the right is blocked with the phospho peptide. Western blot analysis of HeLa cells labelling Lamin A + C (phosphor S392) with ab58528 at 1:1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C (phospho S392) antibody (ab58528)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling Lamin A + C (phospho S392) with ab58528. The image on the right is blocked with the phospho peptide. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Lamin A + C (phosphor S392) with ab58528 at 1:100 dilution.
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Immunocytochemistry - Anti-Lamin A + Lamin C (phospho S392) antibody (ab58528)Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Lamin A + C (phospho S392) with ab58528. The image on the right is blocked with the phospho peptide. Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labelling Lamin A + C (phosphor S392) with ab58528 at 1:200 dilution
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Western blot - Anti-Lamin A + Lamin C (phospho S392) antibody (ab58528)All lanes : Anti-Lamin A + Lamin C (phospho S392) antibody (ab58528) at 1/500 dilution
Lane 1 : Extracts from Hela cells
Lane 2 : Extracts from Hela cells with immunizing peptide
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
