HRP Anti-Lamin A + Lamin C antibody [JOL2] (ab196636)
![HRP Anti-Lamin A + Lamin C antibody [JOL2] (ab196636)](https://www.abcam.com/ps/products/196/ab196636/Images/ab196636-386669-anti-lamin-a-lamin-c-antibody-jol2-hrp-western-blot-hap1-wt-hap1-lmna-knockout-hela-hepg2.jpg "HRP Anti-Lamin A + Lamin C antibody [JOL2] (ab196636)")
Key features and details
- HRP Mouse monoclonal [JOL2] to Lamin A + Lamin C
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human, African green monkey
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-Lamin A + Lamin C antibody [JOL2]
See all Lamin A + Lamin C primary antibodies -
Description
HRP Mouse monoclonal [JOL2] to Lamin A + Lamin C -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Lamin A + Lamin C.
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Epitope
This product has been shown to bind to an epitope between amino acids 464-572. -
Positive control
- WB: HAP1, HeLa and HepG2 cell lysate IHC-P: Human young placenta, tonsil.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
JOL2 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Images
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Western blot - HRP Anti-Lamin A + Lamin C antibody [JOL2] (ab196636)All lanes : HRP Anti-Lamin A + Lamin C antibody [JOL2] (ab196636) at 1/200 dilution
Lane 1 : Wild-type HAP1 cell lysate at 40 µg
Lane 2 : LMNA knockout HAP1 cell lysate at 40 µg
Lane 3 : HeLa cell lysate at 20 µg
Lane 4 : HepG2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 70-75 kDa why is the actual band size different from the predicted?
Exposure time: 150 secondsab196636 was shown to react with Anti-Lamin A + C antibody [JOL2] (HRP) in wild-type HAP1 cells in Western blot. Loss of signal was observed when LMNA knockout sample was used. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab196636 overnight at 4°C at a 1 in 200 dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor® 680) at a 1 in 1000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Lamin A + Lamin C antibody [JOL2] (ab196636)](https://www.abcam.com/ps/products/196/ab196636/Images/ab196636-234004-anti-lamin-a-c-antibody-jol2-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Lamin A + Lamin C antibody [JOL2] (ab196636)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labelling Lamin A + C with ab196636.
