Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FAK antibody [63D5] (ab72140)

Key features and details

  • Mouse monoclonal [63D5] to FAK
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-FAK antibody [63D5]
    See all FAK primary antibodies

  • Description

    Mouse monoclonal [63D5] to FAK

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment corresponding to amino acids 706-1052 of Human FAK with a His tag

  • Positive control

    • NIH 3T3 cytoplasmic extract lysate (ab14873) can be used as a positive control in WB. A431, HeLa, NIH2T3 and C6 cell lysates

  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR188357 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-FAK antibody [63D5] (ab72140)
    Flow Cytometry - Anti-FAK antibody [63D5] (ab72140)
    Overlay histogram showing HeLa cells stained with ab72140 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72140, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot - Anti-FAK antibody [63D5] (ab72140)
    Western blot - Anti-FAK antibody [63D5] (ab72140)
    All lanes : Anti-FAK antibody [63D5] (ab72140) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : NIH3T3 cell lysate
    Lane 4 : C6 cell lysate

    Predicted band size: 119 kDa
    Observed band size: 119 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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