HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836)
![HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836)](https://www.abcam.com/ps/products/201/ab201836/Images/ab201836-312037-ab201836KOWBJRIZ.jpg "HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR6099] to Insulin degrading enzyme / IDE
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099]
See all Insulin degrading enzyme / IDE primary antibodies -
Description
HRP Rabbit monoclonal [EPR6099] to Insulin degrading enzyme / IDE -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2, A375, and K562 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR6099 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836)All lanes : HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : IDE (Insulin degrading enzyme / IDE) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Exposure time: 20 minutesab201836 was shown to specifically react with Insulin degrading enzyme / IDE in wild-type HAP1 cells as signal was lost in IDE (Insulin degrading enzyme / IDE) knockout cells. Wild-type and IDE (Insulin degrading enzyme / IDE) knockout samples were subjected to SDS-PAGE. Ab201836 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836)](https://www.abcam.com/ps/products/201/ab201836/Images/ab201836-246424-WBab2018361.jpg)
Western blot - HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836)All lanes : HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : A375 (Human melanoma cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201836 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836)](https://www.abcam.com/ps/products/201/ab201836/Images/ab201836-4-benefits-of-recombinant-antibodies.png)
HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836)
