Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Insulin degrading enzyme / IDE antibody [EPR6099] - BSA and Azide free (ab247897)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6099] to Insulin degrading enzyme / IDE - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Insulin degrading enzyme / IDE antibody [EPR6099] - BSA and Azide free
    See all Insulin degrading enzyme / IDE primary antibodies

  • Description

    Rabbit monoclonal [EPR6099] to Insulin degrading enzyme / IDE - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HAP1, K562 and HepG2 cell lysates.

  • General notes

    Ab247897 is the carrier-free version of ab109538. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247897 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6099] - BSA and Azide free (ab247897)
    Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6099] - BSA and Azide free (ab247897)
    All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab109538) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : IDE knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 118 kDa
    Observed band size: 118 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab109538).

      Lanes 1- 2: Merged signal (red and green). Green - ab109538 observed at 118 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab109538 was shown to react with IDE in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261755 (knockout cell lysate ab257197) was used. Wild-type HeLa and IDE knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109538 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6099] - BSA and Azide free (ab247897)
    Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6099] - BSA and Azide free (ab247897)
    All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab109538) at 1/2000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : IDE knockout HAP1 cell lysate,
    Lane 3 : K562 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 118 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab109538).

    Lanes 1 - 4: Merged signal (red and green). Green - ab109538 observed at 118 kDa. Red - loading control, ab8245, observed at 37 kDa.


    ab109538 was shown to specifically react with IDE in wild-type HAP1 cells. No band was observed when IDE knockout samples were examined. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab109538 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Anti-Insulin degrading enzyme / IDE antibody [EPR6099] - BSA and Azide free (ab247897)
    Anti-Insulin degrading enzyme / IDE antibody [EPR6099] - BSA and Azide free (ab247897)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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