Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RPS24 antibody [EPR16017(B)] (ab196652)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16017(B)] to RPS24
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-RPS24 antibody [EPR16017(B)]
See all RPS24 primary antibodies
Description

Rabbit monoclonal [EPR16017(B)] to RPS24
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Jurkat and 293T whole cell lysates; Mouse colon, rat colon and mouse liver lysates; Human squamous cell carcinoma of cervix, Human tonsil and mouse liver tissues; SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16017(B)
Isotype

IgG
Research areas

Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652)

All lanes : Anti-RPS24 antibody [EPR16017(B)] (ab196652) at 1/5000 dilution

Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652)

All lanes : Anti-RPS24 antibody [EPR16017(B)] (ab196652) at 1/1000 dilution

Lane 1 : Mouse colon lysate
Lane 2 : Rat colon lysate
Lane 3 : Mouse liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)

Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human squamous cell carcinoma of cervix tissue is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-RPS24 antibody [EPR16017(B)] (ab196652)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling RPS24 with ab196652 at 1/250 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on SH-SY5Y cell line is observed. The nuclear counter stain is DAPI (blue).
Anti-RPS24 antibody [EPR16017(B)] (ab196652)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com