Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RPS24 antibody [EPR16017(B)] (ab196652)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16017(B)] to RPS24
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RPS24 antibody [EPR16017(B)]
    See all RPS24 primary antibodies

  • Description

    Rabbit monoclonal [EPR16017(B)] to RPS24

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat and 293T whole cell lysates; Mouse colon, rat colon and mouse liver lysates; Human squamous cell carcinoma of cervix, Human tonsil and mouse liver tissues; SH-SY5Y cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16017(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
    Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
    All lanes : Anti-RPS24 antibody [EPR16017(B)] (ab196652) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
    Western blot - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
    All lanes : Anti-RPS24 antibody [EPR16017(B)] (ab196652) at 1/1000 dilution

    Lane 1 : Mouse colon lysate
    Lane 2 : Rat colon lysate
    Lane 3 : Mouse liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)

    Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human squamous cell carcinoma of cervix tissue is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS24 antibody [EPR16017(B)] (ab196652)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS24 antibody [EPR16017(B)] (ab196652)
    Immunocytochemistry/ Immunofluorescence - Anti-RPS24 antibody [EPR16017(B)] (ab196652)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling RPS24 with ab196652 at 1/250 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on SH-SY5Y cell line is observed. The nuclear counter stain is DAPI (blue).

  • Anti-RPS24 antibody [EPR16017(B)] (ab196652)
    Anti-RPS24 antibody [EPR16017(B)] (ab196652)

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