Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Wednesday March 03, 2021

Anti-RPE65 antibody [EPR22579-44] - BSA and Azide free (ab254247)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22579-44] to RPE65 - BSA and Azide free
Suitable for: WB, IHC-P, IHC-Fr, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-RPE65 antibody [EPR22579-44] - BSA and Azide free
See all RPE65 primary antibodies
Description

Rabbit monoclonal [EPR22579-44] to RPE65 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IHC-Fr, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human retina tissue; mouse retina tissue; rat retina tissue. IHC-Fr: Mouse retina tissue; rat retina tissue. IP: Mouse eyeball lysate.
General notes
Ab254247 is the carrier-free version of ab231782. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab254247 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22579-44
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-RPE65 antibody [EPR22579-44] (ab254247)

Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized rat retinal tissue labeling RPE65 with ab231782 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining on retinal pigment epithelium (PMID: 17848510) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231782).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPE65 antibody [EPR22579-44] (ab254247)

Immunohistochemical analysis of paraffin-embedded human retina tissue labeling RPE65 with ab231782 at 1/4000 dilution, followed by ready to use AP-labeled secondary antibody kit. Cytoplasmic staining on pigment epithelium cells of human retina (PMID: 25941382; PMID: 22238088) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use AP-labeled secondary antibody kit.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab231782 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231782).
Immunoprecipitation - Anti-RPE65 antibody [EPR22579-44] (ab254247)

RPE65 was immunoprecipitated from 0.35 mg of mouse eyeball lysate with ab231782 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab231782 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1: Mouse eyeball lysate 10 μg (Input).
Lane 2: ab231782 IP in mouse eyeball lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab231782 in mouse eyeball lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231782).
Immunohistochemistry (Frozen sections) - Anti-RPE65 antibody [EPR22579-44] (ab254247)

Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized mouse retinal tissue labeling RPE65 with ab231782 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining on retinal pigment epithelium (PMID: 17848510) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231782).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPE65 antibody [EPR22579-44] (ab254247)

Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling RPE65 with ab231782 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on pigment epithelium cells of rat retina (PMID: 25941382, PMID: 22238088) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231782).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPE65 antibody [EPR22579-44] (ab254247)

Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling RPE65 with ab231782 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on pigment epithelium cells of mouse retina (PMID: 25941382, PMID: 22238088 is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231782).
Anti-RPE65 antibody [EPR22579-44] - BSA and Azide free (ab254247)

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