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Anti-Dkk3 antibody [EPR15611] - BSA and Azide free (ab232657)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15611] to Dkk3 - BSA and Azide free
  • Suitable for: ICC, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dkk3 antibody [EPR15611] - BSA and Azide free
    See all Dkk3 primary antibodies

  • Description

    Rabbit monoclonal [EPR15611] to Dkk3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HepG2 cells.

  • General notes

    Ab232657 is the carrier-free version of ab186409. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232657 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] - BSA and Azide free (ab232657)
    Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] - BSA and Azide free (ab232657)

    Western blot analysis of Dkk3 in Human fetal brain lysate immunoprecipitated with ab186409 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal brain lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186409).

  • Immunocytochemistry - Anti-Dkk3 antibody [EPR15611] - BSA and Azide free (ab232657)
    Immunocytochemistry - Anti-Dkk3 antibody [EPR15611] - BSA and Azide free (ab232657)

    Clone EPR15611 (ab232657) has been successfully conjugated by Abcam. This image was generated using Anti-Dkk3 antibody [EPR15611] (Alexa Fluor® 488). Please refer to ab201262 for protocol details.

    ab201262 staining Dkk3 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201262 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry - Anti-Dkk3 antibody [EPR15611] - BSA and Azide free (ab232657)
    Immunocytochemistry - Anti-Dkk3 antibody [EPR15611] - BSA and Azide free (ab232657)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Dkk3 with ab186409 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186409).

  • Anti-Dkk3 antibody [EPR15611] - BSA and Azide free (ab232657)
    Anti-Dkk3 antibody [EPR15611] - BSA and Azide free (ab232657)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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