Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3] (ab278716)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MKK3S189MKK6S207-D3] to MEK3 + MKK6
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3]

  • Description

    Rabbit monoclonal [MKK3S189MKK6S207-D3] to MEK3 + MKK6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MEK3 (phospho S189). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P46734

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: UV-treated COS-7 cell extracts. Flow Cyt: HEK-293T cells treated with UV+TPA. NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3] (ab278716)
    Western blot - Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3] (ab278716)
    All lanes : Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3] (ab278716) at 0.05 µg/ml

    Lane 1 : Untreated COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts
    Lane 2 : UV-treated COS-7 cell extracts
  • Flow Cytometry - Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3] (ab278716)
    Flow Cytometry - Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3] (ab278716)

    Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells secondary antibody only negative control (blue) or treated with K252a (red) or treated with UV+TPA (green) using ab278716 at 0.5 µg/ml.

  • Flow Cytometry - Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3] (ab278716)
    Flow Cytometry - Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3] (ab278716)

    Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells secondary antibody only (blue) or 0.1 µg/ml of isotype control (orange) or ab278716.

  • Flow Cytometry - Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3] (ab278716)
    Flow Cytometry - Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3] (ab278716)

    Peptide blocking flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells secondary antibody only negative control (light blue) or treated with K252a (red) or UV/TPA-treated (green) or K252a and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or K252a and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using ab278716 at 0.1 µg/ml.

  • Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3] (ab278716)
    Anti-MEK3 + MKK6 antibody [MKK3S189MKK6S207-D3] (ab278716)

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