Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21732] to CD130 (gp130) - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free
    See all CD130 (gp130) primary antibodies

  • Description

    Rabbit monoclonal [EPR21732] to CD130 (gp130) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, HeLa, HAP1, and PC-3 whole cell lysates.

  • General notes

    ab234105 is the carrier-free version of ab217671. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab234105 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)
    Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)
    All lanes : Anti-CD130 (gp130) antibody [EPR21732] (ab217671) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : IL6ST knockout A549 cell lysate
    Lane 3 : PC3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 104 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab217671).

    Lanes 1- 2: Merged signal (red and green). Green - ab217671 observed at 130 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab217671 was shown to react with CD130 (gp130) in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line ab266939 (knockout cell lysate ab257208) was used. Wild-type A549 and IL6ST knockout A549 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab217671 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • ELISA - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)
    ELISA - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)

    This data was developed using ab217671, the same antibody clone in a different buffer formulation.

    ELISA analysis of IL6ST recombinant protein at 1000 ng/mL with ab217671. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)
    Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)
    All lanes : Anti-CD130 (gp130) antibody [EPR21732] (ab217671) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : IL6ST knockout A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 104 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab217671).

    Lanes 1-2: Merged signal (red and green). Green - ab217671 observed at 130 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab217671 Anti-CD130 (gp130) antibody [EPR21732] was shown to specifically react with CD130 (gp130) in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266938 (knockout cell lysate ab257207) was used. Wild-type and CD130 (gp130) knockout samples were subjected to SDS-PAGE. ab217671 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)
    Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)
    All lanes : Anti-CD130 (gp130) antibody [EPR21732] (ab217671) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : IL6ST knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 104 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab217671).

    Lanes 1 - 2: Merged signal (red and green). Green - ab217671 observed at 130 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab217671 Anti-CD130 (gp130) antibody [EPR21732] was shown to specifically react with CD130 (gp130) in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265767 (knockout cell lysate ab257205) was used. Wild-type and CD130 (gp130) knockout samples were subjected to SDS-PAGE. ab217671 and Anti-GAPDH antibody [6C5] - Loading Control were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)
    Western blot - Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)
    All lanes : Anti-CD130 (gp130) antibody [EPR21732] (ab217671) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 (human chronic myelogenous leukemia cell line) whole cell lysate
    Lane 2 : CD130 (gp130) knockout HAP1 whole cell lysate
    Lane 3 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 104 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?


    Exposure time: 114 seconds


    locking and dilution buffer: 5% NFDM/TBST.

    ab217671 was shown to specifically react with CD130 (gp130) in wild-type HAP1 cells as the signal was lost in CD130 (gp130) knockout cells. Wild-type and CD130 (gp130) knockout samples were subjected to SDS-PAGE. ab217671 and ab181602 (Human anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/200000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217671).

  • Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)
    Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)

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