Cell Transformation Assay Kit (Colorimetric) (ab235698)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cell Transformation Assay Kit (Colorimetric)
See all Cell Transformation kits -
Detection method
Colorimetric -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Anchorage-independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). Cell Transformation Assay Kit (Colorimetric)(ab235698) is faster, stable, and more sensitive than the traditional Soft-Agar Assay. The kit is based on the conversion of the tetrazolium salt (WST) to formazan by cellular mitochondrial dehydrogenases. The generated signal is directly proportional to the number of living cells. This one-step method is non-radioactive and simple (just add-and-read, does not require harvesting cells, and solubilization steps). The assay is high-throughput adaptable and has a wide linear range from 10,000-400,000 cells. The entire Cell Transformation Assay can be finished within 7-8 days.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Agarose Powder 1 x 240mg DMEM Solution (10X) 2 x 1.5ml Electro Coupling Solution (ECS) 1 x 1.8ml Staining Solution 1 x 1ml WST Reagent 1 vial
Images
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Typical assay data
(A) HeLa (human epithelial cell line from cervix adenocarcinoma) cell-dose curve; (B) Equal numbers of NIH/3T3 (mouse embryo fibroblast cell line) and HeLa cells were seeded for the Cell Transformation Assay. After 6 days, the cell numbers were measured. Standard and Samples readings were taken 4 hours after adding WST Working Solution; (C) Image of HeLa cell colonies. HeLa cells were cultured for 6 days according to the kit protocol.
