Cell Transformation Assay Kit (Fluorometric) (ab235699)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cell Transformation Assay Kit (Fluorometric)
See all Cell Transformation kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Species reactivity
Predicted to work with: Mammals
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Product overview
Transformed cells can proliferate without attaching to a surface. Anchorage-independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). The Cell Transformation Assay Kit (Fluorimetric) (ab235699) is faster, stable and more sensitive than the traditional Soft-Agar Assay. The kit uses a quantitative dye that binds to nucleic acid and generates green fluorescence. This one-step method is non-radioactive and simple (just add-and-read, and does not require tedious labor such as counting colonies). The assay is high-throughput adaptable and has wide linear range from 50-60,000 cells. The entire assay can be finished within 7-8 days.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Agarose Powder 1 x 240mg Agarose Solubilization Solution 1 x 5ml DMEM Solution (10X) 2 x 1.5ml Quantitative Dye (200X) 1 x 0.1ml Staining Solution 1 x 1ml
Images
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Typical assay data
(a) HeLa (Human epithelial cell line from cervix adenocarcinoma) cell-dose curve; (b) Equal numbers of NIH/3T3 (Mouse embryo fibroblast cell line) and HeLa cells were serially diluted and seeded in agarose gel. Cells were solubilized and detected with Quantitative Dye; (c) Image of HeLa cell colonies. HeLa cells were cultured for 7 days according to the kit protocol.
