Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23991-53] to Interferon gamma - BSA and Azide free
  • Suitable for: ICC, IP, WB, Flow Cyt (Intra)
  • Reacts with: Human

Overview

  • Product name

    Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free
    See all Interferon gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR23991-53] to Interferon gamma - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WB, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NK-92 lysates treated with 80nM PMA and 3µM Ionomycin for 5 hours, then with 300 ng/ml Brefeldin A (BFA) for 1 hour. ICC: NK-92 cells. Flow Cyt (intra): PBMC cells treated with cell stimulation cocktail (80nM PMA+1.34µM Ionomycin+10.6µM BFA+2uM Monensin), NK-92 treated with PMA, 80 nM and lonomycin (3µM) for 1h then co-treated with BFA (300 ng/ml) for 4h. IP: NK-92 treated with 80nM PMA and 3µM Ionomycin for 5 h, then with 300 ng/ml BFA cell.

  • General notes

    ab280574 is the carrier-free version of ab267369. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280574 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23991-53

  • Isotype

    IgG

Images

  • Western blot - Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)
    Western blot - Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)
    All lanes : Anti-Interferon gamma antibody [EPR23991-53] (ab267369) at 1/1000 dilution

    Lane 1 : Untreated NK-92 (human malignant non-hodgkins lymphoma natural killer cell) whole cell lysate
    Lane 2 : NK-92 treated with 80nM PMA and 3uM Ionomycin for 5 hours, then with /ml Brefeldin A (BFA) added after 1 hour, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 19 kDa



    This data was developed using ab267369, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight  observed is consistent with what has been described in the literature (PMID:23129404)

    Exposure time: 92 seconds

  • Immunocytochemistry - Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)
    Immunocytochemistry - Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)

    This data was developed using ab267369, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NK-92 cells labelling Interferon gamma with ab267369 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in NK-92 treated with phorbol 12-myristate 13-acetate (80 nM) and lonomycin (3 µM) for 1 hour and then treated with brefeldin A (300 ng/ml) for another 4 hours is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)
    Flow Cytometry - Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)

    This data was developed using ab267369, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell) treated with phorbol 12-myristate 13-acetate (PMA, 80 nM) and lonomycin (3 µM) for 1h and then co-treated with brefeldin A (300 ng/ml) for another 4h cells labelling Interferon gamma with ab267369 at 1/500 dilution (0.1ug) (Right) / compared with Untreated control cells (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)
    Flow Cytometry - Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)

    This data was developed using ab267369, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) treated with cell stimulation cocktail (80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours cells labelling Interferon gamma with ab267369 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD4 conjugated to Pacific blue. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or ab267369.

  • Immunoprecipitation - Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)
    Immunoprecipitation - Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)

    This data was developed using ab267369, the same antibody clone in a different buffer formulation.

    Interferon gamma was immunoprecipitated from 0.35 mg NK-92 treated with 80nM PMA and 3µM Ionomycin for 5 hours, then with 300 ng/ml Brefeldin A (BFA) added after 1 hour, whole cell lysate 100 ug with ab267369 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267369 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NK-92 treated with 80nM PMA and 3µM Ionomycin for 5 hours, then with 300 ng/ml Brefeldin A (BFA) added after 1 hour, whole cell lysate 100 ug

    Lane 2: ab267369 IP in NK-92 treated with 80nM PMA and 3µM Ionomycin for 5 hours, then with 300 ng/ml Brefeldin A (BFA) added after 1 hour, whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267369 in NK-92 treated with 80nM PMA and 3µM Ionomycin for 5 hours, then with 300 ng/ml Brefeldin A (BFA) added after 1 hour, whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

     

  • Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)
    Anti-Interferon gamma antibody [EPR23991-53] - BSA and Azide free (ab280574)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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