Anti-sRANKL antibody (ab62516)
Key features and details
- Rabbit polyclonal to sRANKL
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-sRANKL antibody
See all sRANKL primary antibodies -
Description
Rabbit polyclonal to sRANKL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (Human) of 14 amino acids from near the centre of sRANKL
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General notes
This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-sRANKL antibody (ab62516) at 1 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 35 kDa
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All lanes : Anti-sRANKL antibody (ab62516) at 2 µg/ml
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse skin tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 35 kDa
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Lane 1 : Anti-sRANKL antibody (ab62516) at 0.25 µg/ml
Lane 2 : Anti-sRANKL antibody (ab62516) at 0.5 µg/ml
All lanes : rat liver tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 35 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
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Immunohistochemical analysis of RANKL expression in paraffin embedded human liver tissue using 5 µg/ml ab62516