Biotin Anti-sRANKL antibody (ab83145)
Key features and details
- Biotin Rabbit polyclonal to sRANKL
- Suitable for: Sandwich ELISA, WB
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-sRANKL antibody
See all sRANKL primary antibodies -
Description
Biotin Rabbit polyclonal to sRANKL -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA Recombinant fragmentWB Recombinant fragment
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Immunogen
Highly pure (>98%) recombinant human sRANKL.
Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Reconstitute in 50 µl sterile PBS containing 0.1% BSA to give a concentration of 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab83145 is sterile filtered. ab83145 was purified by affinity chromatography and then biotinylated. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Biotin Anti-sRANKL antibody (ab83145)
To detect human sRANKL by Western Blot analysis ab83145 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human sRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Human recombinant sRANKL protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant sRANKL protein.
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Sandwich ELISA - Biotin Anti-sRANKL antibody (ab83145)To detect human sRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83145 is required. This biotinylated polyclonal antibody, in conjunction with an anti-RANKL (ab9957) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human sRANKL.
