Lipid Peroxidation (4-HNE) Assay Kit (ab238538)
Key features and details
- Assay type: Competitive
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Purified protein, Serum, Tissue Lysate
Overview
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Product name
Lipid Peroxidation (4-HNE) Assay Kit -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Cell Lysate, Purified protein, Tissue Lysate -
Assay type
Competitive -
Species reactivity
Reacts with: Vertebrata -
Product overview
Lipid Peroxidation (4-HNE) Assay Kit (ab238538) is designed for the rapid detection and quantitation of 4-hydroxynonenal (4-HNE)-protein adducts.
4-HNE is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. This Competitive ELISA kit will allow the quantitation of 4-HNE adduct in protein samples determined by comparing its absorbance with that of a known 4-HNE-BSA standard curve.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 32 tests 100X Conjugate Diluent 1 x 300µl 1 x 100µl 10X Wash Buffer 1 x 100ml 1 x 30ml Protein Binding Strip Well Plate 1 unit 1 unit Anti-HNE Antibody (1000X) 1 x 10µl 1 x 5µl Assay Diluent 1 x 50ml 1 x 20ml HNE Conjugate 1 x 50µl 1 x 20µl HNE-BSA Standard 1 x 250µl 1 x 100µl Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl 1 x 20µl Stop Solution 1 x 12ml 1 x 4ml Substrate Solution 1 x 12ml 1 x 4ml -
Research areas
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Relevance
Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress. -
Cellular localization
Cytoplasmic -
Alternative names
- 4 HNE
- 4-Hydroxy-2-Nonenal
Images
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HNE-BSA Competitive ELISA Standard Curve.
The following figures demonstrate typical HNE Adduct Competitive ELISA results. One should use the data below for reference only. This data should not be used to interpret actual results.