Human GITR ELISA Kit (ab234571)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 14 pg/ml
- Range: 78 pg/ml - 5000 pg/ml
- Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human GITR ELISA Kit
See all GITR kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% MCF7 Extract 5 3.2% Inter-assay Sample n Mean SD CV% MCF7 Extract 3 4.4% -
Sample type
Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
14 pg/ml -
Range
78 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94 90% - 98% Cell culture extracts 92 87% - 96% Cell culture media 105 103% - 107% Hep Plasma 95 92% - 101% EDTA Plasma 93 90% - 96% Cit plasma 89 86% - 91% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human GITR ELISA Kit (ab234571) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GITR protein in cell culture extracts, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GITR with 14 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human GITR Capture Antibody 1 x 600µl 10X Human GITR Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human GITR Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. -
Tissue specificity
Expressed in lymph node, peripheral blood leukocytes and weakly in spleen. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- Activation inducible TNFR family receptor
- Activation-inducible TNFR family receptor
- AITR
see all -
Database links
- Entrez Gene: 8784 Human
- Omim: 603905 Human
- SwissProt: Q9Y5U5 Human
- Unigene: 212680 Human
Images
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Other - Human GITR ELISA Kit (ab234571)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human GITR standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human GITR standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked GITR in human serum, plasma and cell culture supernatant samples.The concentrations of GITR were measured in duplicates, interpolated from the GITR standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 25%, plasma (citrate) 25%, plasma (heparin) 25% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native GITR in human MCF7 cell extract based on a 1,000 µg/mL extract load.The concentrations of GITR were measured in duplicate and interpolated from the GITR standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GITR concentration was determined to be 757 pg/mL in MCF7 cell extract.
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Sandwich ELISA - Human GITR ELISA Kit (ab234571)To learn more about the advantages of recombinant antibodies see here.
