Anti-Firefly Luciferase antibody [EPR17789] - BSA and Azide free (ab238448)
![Anti-Firefly Luciferase antibody [EPR17789] - BSA and Azide free (ab238448)](https://www.abcam.com/ps/products/238/ab238448/Images/ab238448-2-ab185923274483185923FC1.jpg "Anti-Firefly Luciferase antibody [EPR17789] - BSA and Azide free (ab238448)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17789] to Firefly Luciferase - BSA and Azide free
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Firefly
Overview
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Product name
Anti-Firefly Luciferase antibody [EPR17789] - BSA and Azide free
See all Firefly Luciferase primary antibodies -
Description
Rabbit monoclonal [EPR17789] to Firefly Luciferase - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt FireflyICC/IF Firefly
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Firefly Luciferase transfected HEK-293T cells.
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General notes
Ab238448 is the carrier-free version of ab185923. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab238448 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17789 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17789] - BSA and Azide free (ab238448)
Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) transfected with GFP tagged Firefly Luciferase cells labeling Firefly Luciferase with purified ab185923 at 1/200 dilution (10ug/ml, Right panel). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185923).
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![Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17789] - BSA and Azide free (ab238448)](https://www.abcam.com/ps/products/238/ab238448/Images/ab238448-319778-448.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17789] - BSA and Azide free (ab238448)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Firefly Luciferase, labeling Firefly Luciferase with ab185923 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK293T cells transfected with Firefly Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab185923 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185923).
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![Anti-Firefly Luciferase antibody [EPR17789] - BSA and Azide free (ab238448)](https://www.abcam.com/ps/products/238/ab238448/Images/ab238448-3-benefits-of-recombinant-antibodies.png)
Anti-Firefly Luciferase antibody [EPR17789] - BSA and Azide free (ab238448)
