Anti-Firefly Luciferase antibody (ab181640)
Key features and details
- Goat polyclonal to Firefly Luciferase
- Suitable for: WB
- Reacts with: Firefly
- Isotype: IgG
Overview
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Product name
Anti-Firefly Luciferase antibody
See all Firefly Luciferase primary antibodies -
Description
Goat polyclonal to Firefly Luciferase -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Firefly
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Immunogen
Full length native protein (purified) corresponding to Firefly (Photinus pyralis) Firefly Luciferase aa 1-550.
Sequence:MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVN ITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGV AVAPANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKII IMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSG STGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMF TTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTL IDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAIL ITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVD YVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL
Database link: P08659 -
Positive control
- Luciferase protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab181640 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. ab181640 is sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Firefly Luciferase antibody (ab181640)All lanes : Anti-Firefly Luciferase antibody (ab181640) at 1/1000 dilution
Lane 1 : Non-transfected 293 cell lysate
Lane 2 : Firefly Luciferase transfected 293 cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : NIH3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (ab216775) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green – ab181640 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab181640 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) at a 1:10000 dilution for 1hr at room temperature and then imaged.
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Western blot - Anti-Firefly Luciferase antibody (ab181640)Anti-Firefly Luciferase antibody (ab181640) at 1/1000 dilution + Luciferase protein at 0.05 µg
Secondary
Peroxidase anti-goat secondary antibody at 1/40000 dilution
Predicted band size: 61 kDa
