Anti-Firefly Luciferase antibody (ab21176)
Key features and details
- Rabbit polyclonal to Firefly Luciferase
- Suitable for: ICC/IF, WB
- Reacts with: Firefly
- Isotype: IgG
Overview
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Product name
Anti-Firefly Luciferase antibody
See all Firefly Luciferase primary antibodies -
Description
Rabbit polyclonal to Firefly Luciferase -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Firefly -
Immunogen
Full length native protein (purified) (Firefly (Photinus pyralis)).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (ab21176)
ab21176 at 10 µg/mL staining Luciferase in transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells by ICC/IF.
The cells were fixed with methanol and acetone. An FITC conjugated anti-Rabbit IgG was used as the secondary antibody.
Left panel: Un-transfected cells.
Right panel: Transfected cells.
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Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (ab21176) This image is courtesy of an Abreview submitted by Dr Mal Niladriab21176 at 1/200 dilution staining engineered adult rat stromal stem cells by ICC/IF.
The cells were fixed in 2% paraformaldehyde and 0.1% Triton X-100 was used for cell permeabilization (15 minutes incubation time). The cells were incubated with the antibody overnight at 4°C. The image shows Luciferase (green-upper right panel), counterstained cell nuclei (DAPI-blue-upper left panel), overlay (lower left panel) and a phase contrast image (lower right panel).
The image was taken with a confocal laser scanning microscope equipped with an additional laser differential Interference Contrast (DIC) mode.
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Western blot - Anti-Firefly Luciferase antibody (ab21176)All lanes : Anti-Firefly Luciferase antibody (ab21176) at 1/1000 dilution
Lane 1 : Lysates from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells overexpressing luciferase
Lane 2 : Lysates from HEK-293T cells overexpressing luciferase with Luciferase Immunizing Peptide
Secondary
All lanes : Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate
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Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (ab21176)Immunocytochemical analysis analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells overexpressing luciferase labeling Luciferase with ab21176 at a concentration of 10 μg/mL. The secondary antibody was a Goat anti-Rabbit IgG, FITC conjugate.
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Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (ab21176) This image is courtesy of an anonymous Abreviewab21176 staining mouse mammary carcinoma cells by ICC/IF.
Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking with a commercial blocking agent. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. An Alexa-Fluor® 555 conjugated goat anti-rabbit antibody was used as the secondary.
The image shows firefly luciferase (red) in mouse tumour cells and cell nuclei counterstained with Hoechst (blue).
