Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-SGTB/SGT2 antibody [EPR17183] - BSA and Azide free
See all SGTB/SGT2 primary antibodies
Description

Rabbit monoclonal [EPR17183] to SGTB/SGT2 - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
ab251369 is the carrier-free version of ab202419 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251369 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product was previously labelled as SGTB
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR17183
Isotype

IgG
Research areas

Western blot - Anti-SGTB/SGT2 antibody [EPR17183] - BSA and Azide free (ab251369)

Anti-SGTB/SGT2 antibody [EPR17183] (ab202419) at 1/2000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa
Observed band size: 37 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab202419, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature, PMID: 12878599.
Western blot - Anti-SGTB/SGT2 antibody [EPR17183] - BSA and Azide free (ab251369)

Anti-SGTB/SGT2 antibody [EPR17183] (ab202419) at 1/2000 dilution + Human fetal brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab202419, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature, PMID: 12878599.
Western blot - Anti-SGTB/SGT2 antibody [EPR17183] - BSA and Azide free (ab251369)

All lanes : Anti-SGTB/SGT2 antibody [EPR17183] (ab202419) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab202419, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature, PMID: 12878599.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGTB/SGT2 antibody [EPR17183] - BSA and Azide free (ab251369)

This data was developed using ab202419, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SGTB/SGT2 with ab202419 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGTB/SGT2 antibody [EPR17183] - BSA and Azide free (ab251369)

This data was developed using ab202419, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling SGTB/SGT2 with ab202419 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on mouse cerebral cortex tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGTB/SGT2 antibody [EPR17183] - BSA and Azide free (ab251369)

This data was developed using ab202419, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling SGTB/SGT2 with ab202419 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on rat cerebral cortex tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-SGTB/SGT2 antibody [EPR17183] - BSA and Azide free (ab251369)

This data was developed using ab202419, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling SGTB/SGT2 with ab202419 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on SH-SY5Y cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red). The negative controls are as follows:
-ve control 1: ab202419 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunocytochemistry - Anti-SGTB/SGT2 antibody [EPR17183] - BSA and Azide free (ab251369)

This data was developed using ab202419, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling SGTB/SGT2 with ab202419 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and cytoplasmic staining on U-87 MG cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red). The negative controls are as follows:
-ve control 1: ab202419 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunoprecipitation - Anti-SGTB/SGT2 antibody [EPR17183] - BSA and Azide free (ab251369)

This data was developed using ab202419, the same antibody clone in a different buffer formulation.SGTB/SGT2 was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab202419 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab202419 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Mouse brain whole cell lysate 10 µg (Input). Lane 2: ab202419 IP in Mouse brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202419 in Mouse brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
Anti-SGTB/SGT2 antibody [EPR17183] - BSA and Azide free (ab251369)