Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CEACAM1 antibody [11-1H] (ab254156)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [11-1H] to CEACAM1
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Rat

Overview

  • Product name

    Anti-CEACAM1 antibody [11-1H]
    See all CEACAM1 primary antibodies

  • Description

    Mouse monoclonal [11-1H] to CEACAM1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Rat
    IHC-P
    Rat
    WB
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment within Rat CEACAM1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P16573

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat liver lysate. IHC-P: Rat liver tissue. IHC-Fr: Rat liver tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    11-1H

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Frozen sections) - Anti-CEACAM1 antibody [11-1H] (ab254156)
    Immunohistochemistry (Frozen sections) - Anti-CEACAM1 antibody [11-1H] (ab254156)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling CEACAM1 with ab254156 at 5.62 µg/mL followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution (Green). Positive staining on rat liver is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-CEACAM1 antibody [11-1H] (ab254156)
    Western blot - Anti-CEACAM1 antibody [11-1H] (ab254156)
    Anti-CEACAM1 antibody [11-1H] (ab254156) at 0.562 µg/ml + Rat liver tissue lysate at 10 µg

    Secondary
    Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 57 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [11-1H] (ab254156)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [11-1H] (ab254156)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling CEACAM1 with ab254156 at 2.81 µg/mL followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat liver. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-CEACAM1 antibody [11-1H] (ab254156)
    Anti-CEACAM1 antibody [11-1H] (ab254156)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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