Anti-MEK2 (phospho T394) antibody (ab30622)
Key features and details
- Rabbit polyclonal to MEK2 (phospho T394)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MEK2 (phospho T394) antibody
See all MEK2 primary antibodies -
Description
Rabbit polyclonal to MEK2 (phospho T394) -
Host species
Rabbit -
Specificity
ab30622 recognises endogenous levels of MEK2 only when phosphorylated at Threonine 394. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MEK2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MEK2 (phospho T394) antibody (ab30622)All lanes : Anti-MEK2 (phospho T394) antibody (ab30622) at 1/500 dilution
Lane 1 : extracts from ovary cancer cells.
Lane 2 : extracts from ovary cancer cells, preincubated with synthesized non phosphopeptide
Lane 3 : extracts from ovary cancer cells, preincubated
with synthesized phosphopeptide (negative control).
Predicted band size: 44 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 (phospho T394) antibody (ab30622)Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue sections, using 1/50 MEK2 (Phospho Thr394) Antibody (ab30622). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).
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Western blot - Anti-MEK2 (phospho T394) antibody (ab30622)Anti-MEK2 (phospho T394) antibody (ab30622) at 1/2000 dilution + VEC cell lysates
Predicted band size: 44 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-MEK2 (phospho T394) antibody (ab30622)ICC/IF image of ab30622 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30622, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
