Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-splicing factor 1 antibody [EPR22437-50] - BSA and Azide free
See all splicing factor 1 primary antibodies
Description

Rabbit monoclonal [EPR22437-50] to splicing factor 1 - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Mouse
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC/IF: HeLa and Neuro-2a cells. IHC-P: Human testis and stomach tissue. Mouse and rat testis tissue. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
General notes
Ab254199 is the carrier-free version of ab223256. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab254199 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22437-50
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-splicing factor 1 antibody [EPR22437-50] - BSA and Azide free (ab254199)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Steroidogenic Factor 1/SF-1 with ab223256 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red).

Secondary antibody only control: Used PBS insread of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223256).
Immunocytochemistry/ Immunofluorescence - Anti-splicing factor 1 antibody [EPR22437-50] - BSA and Azide free (ab254199)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling Steroidogenic Factor 1/SF-1 with ab223256 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in Neuro-2a cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223256).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] - BSA and Azide free (ab254199)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Steroidogenic Factor 1/SF-1 with ab223256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis (PMID: 25145264) is observed. The section was incubated with ab223256 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223256).
Flow Cytometry - Anti-splicing factor 1 antibody [EPR22437-50] - BSA and Azide free (ab254199)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling Steroidogenic Factor 1/SF-1 with ab223256 at 1/500 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223256).
Immunoprecipitation - Anti-splicing factor 1 antibody [EPR22437-50] - BSA and Azide free (ab254199)

Steroidogenic Factor 1/SF-1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab223256 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223256 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab223256 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223256 in HeLa whole cell lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 1 second.

The lower bands below 75kDa represent Splicing factor 1 short isoforms and/or degraded fragments.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223256).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] - BSA and Azide free (ab254199)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Steroidogenic Factor 1/SF-1 with ab223256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis (PMID: 25145264) is observed. The section was incubated with ab223256 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223256).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] - BSA and Azide free (ab254199)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Steroidogenic Factor 1/SF-1 with ab223256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human stomach (PMID: 25145264) is observed. The section was incubated with ab223256 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223256).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] - BSA and Azide free (ab254199)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Steroidogenic Factor 1/SF-1 with ab223256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis (PMID: 25145264; PMID: 20736371) is observed. The section was incubated with ab223256 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223256).
Anti-splicing factor 1 antibody [EPR22437-50] - BSA and Azide free (ab254199)