Mouse MASP1 Antibody Pair - BSA and Azide free (ab253360)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse MASP1 Antibody Pair - BSA and Azide free
See all MASP1 kits -
Assay type
ELISA set -
Range
93.75 pg/ml - 6000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
The Antibody Pair can be used to quantify Mouse MASP1. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab272473), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse MASP1 Capture Antibody (unconjugated) 1 x 100µg Mouse MASP1 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Relevance
MASP is a component of the bactericidal ra-reactive factor rarf which specifically binds to RA and R2 polysaccharides expressed by certain enterobacteria. It triggers the activation of complement cascade by activating the C4 and C2 components. It activates the C4 component by cleaving the alpha-chain of C4. The alternative-splicing product from MASP1 gene is called MASP3. MASP1 is associated with smaller MBL oligomers whereas MASP3 is found on larger oligomers. The substrate of MASP3 is unknown. -
Cellular localization
Secreted -
Alternative names
- Complement factor MASP-3
- Complement factor MASP3
- Complement-activating component of Ra-reactive factor
see all -
Database links
- Entrez Gene: 17174 Mouse
- SwissProt: P98064 Mouse
Images
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Sandwich ELISA - Mouse MASP1 Antibody Pair - BSA and Azide free (ab253360)To learn more about the advantages of recombinant antibodies see here.
