Mouse MASP1 ELISA Kit (ab272473)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 29.3 pg/ml
- Range: 93.75 pg/ml - 6000 pg/ml
- Sample type: EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MASP1 ELISA Kit
See all MASP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.2% Inter-assay Sample n Mean SD CV% Serum 3 5.09% -
Sample type
Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
29.3 pg/ml -
Range
93.75 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93 82% - 106% EDTA Plasma 110 109% - 110% Cit plasma 90 78% - 92% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MASP1 ELISA kit (ab272473) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse MASP1 protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MASP1 with 29.3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
MASP1, encoded by the Masp1 gene, is a serine protease and the most abundant protease of the lectin pathway. MASP1 plays a central role in complement activation. The binding of mannose binding lectin (MBL) and ficolins to sugar moieties triggers the lectin pathway and leads to the activation of MASP1 that will recognize and neutralize pathogens through patterns of sugar moieties. Studies have shown MASP1 to promote coagulation and take an active role in the inflammation process. Human, rat, cow, and monkey share 90%, 97%, 89%, and 90% sequence homology, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse MASP1 Capture Antibody 1 x 600µl 10X Mouse MASP1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 4BR 1 x 6ml Mouse MASP1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
MASP is a component of the bactericidal ra-reactive factor rarf which specifically binds to RA and R2 polysaccharides expressed by certain enterobacteria. It triggers the activation of complement cascade by activating the C4 and C2 components. It activates the C4 component by cleaving the alpha-chain of C4. The alternative-splicing product from MASP1 gene is called MASP3. MASP1 is associated with smaller MBL oligomers whereas MASP3 is found on larger oligomers. The substrate of MASP3 is unknown. -
Cellular localization
Secreted -
Alternative names
- Complement factor MASP-3
- Complement factor MASP3
- Complement-activating component of Ra-reactive factor
see all -
Database links
- Entrez Gene: 17174 Mouse
- SwissProt: P98064 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse MASP1 standard curve in Sample Diluent NS + 1X EnhancerThe MASP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MASP1 in mouse serum and plasma samples.The concentrations of MASP1 were measured in duplicates, interpolated from the MASP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (citrate) 1:400, and plasma (EDTA) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MASP1 concentration was determined to be 902.1 ng/mL in serum, 416.2 ng/mL in plasma (citrate) and 779.9 ng/mL in plasma (EDTA).
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Sandwich ELISA - Mouse MASP1 ELISA Kit (ab272473)To learn more about the advantages of recombinant antibodies see here.
