Anti-Cdk4 antibody (ab137675)
Key features and details
- Rabbit polyclonal to Cdk4
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cdk4 antibody
See all Cdk4 primary antibodies -
Description
Rabbit polyclonal to Cdk4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig
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Immunogen
Recombinant fragment corresponding to Human Cdk4 aa 148-303.
Database link: P11802 -
Positive control
- WB: NIH/3T3, HEK-293T, HeLa, PC-12 whole cell lysates. Epithelial OVCAR whole cell lysate. ICC/IF: HeLa and MCF7 cells. IHC-P: Human gastric carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody (ab137675)
HeLa cells stained for CDK4 (green) using ab137675 at 1/500 dilution in ICC/IF.
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Western blot - Anti-Cdk4 antibody (ab137675)Anti-Cdk4 antibody (ab137675) at 1/1000 dilution + HeLa whole cell extract at 30 µg
Predicted band size: 34 kDa12% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk4 antibody (ab137675)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue, labelling Cdk4 using ab137675 at 1/500 dilution. -
Western blot - Anti-Cdk4 antibody (ab137675)Anti-Cdk4 antibody (ab137675) at 1/500 dilution + PC-12 whole cell extract at 30 µg
Predicted band size: 34 kDa12% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody (ab137675)MCF7 cells stained for CDK4 (green) using ab137675 at 1/1000 dilution in ICC/IF. Red staining of p21 Cip1 (a nucleus marker) at 1/500 dilution and Hoechst 33342 (blue) staining.
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Western blot - Anti-Cdk4 antibody (ab137675)Anti-Cdk4 antibody (ab137675) at 1/1000 dilution + HEK-293T whole cell lysate at 30 µg
Predicted band size: 34 kDa
12% SDS PAGE -
Western blot - Anti-Cdk4 antibody (ab137675)Anti-Cdk4 antibody (ab137675) at 1/1000 dilution + NIH/3T3 whole cell lysate at 30 µg
Predicted band size: 34 kDa
12% SDS PAGE -
Western blot - Anti-Cdk4 antibody (ab137675) This image is courtesy of an anonymous Abreview.All lanes : Anti-Cdk4 antibody (ab137675) at 1/1000 dilution
Lane 1 : Epithelial OVCAR whole cell lysate - untreated
Lane 2 : Epithelial OVCAR whole cell lysate treated with 100µM Perifosine for 24 hours
Lane 3 : Epithelial OVCAR whole cell lysate treated with 200µM Perifosine for 24 hours
Lysates/proteins at 100 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes10% SDS-PAGE.
Blocked with 5% milk for 12 hours at 4°C.
Incubated with the primary antibody in 2% milk for 12 hours at 4°C.
