Anti-BMPR2 antibody [MM0060-9A10] (ab78422)
Key features and details
- Mouse monoclonal [MM0060-9A10] to BMPR2
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-BMPR2 antibody [MM0060-9A10]
See all BMPR2 primary antibodies -
Description
Mouse monoclonal [MM0060-9A10] to BMPR2 -
Host species
Mouse -
Specificity
ab78422 detects BMPR2. No cross reactivity was found to BMPR1A and BMPR1B. -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human BMPR2 extracellular domain
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Positive control
- IHC-P: Human placenta tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate buffered saline (PBS). -
Clonality
Monoclonal -
Clone number
MM0060-9A10 -
Isotype
IgG1 -
Research areas
Images
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ab78422 at 1/200 dilution, staining BMPR2 in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
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Overlay histogram showing HepG2 cells stained with ab78422 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78422, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.