Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BMPR2 antibody [1F12] (ab130206)

Key features and details

  • Mouse monoclonal [1F12] to BMPR2
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-BMPR2 antibody [1F12]
    See all BMPR2 primary antibodies

  • Description

    Mouse monoclonal [1F12] to BMPR2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Purified recombinant fragment of Human BMPR2 expressed in E. Coli.

  • Positive control

    • Breast, stomach and kidney cancer tissue; tonsil tissues. A431, NIH/3T3, Cos7 and PC-12 cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-BMPR2 antibody [1F12] (ab130206)
    Western blot - Anti-BMPR2 antibody [1F12] (ab130206)
    All lanes : Anti-BMPR2 antibody [1F12] (ab130206) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : NIH/3T3 cell lysate
    Lane 4 : Cos7 cell lysate
    Lane 5 : PC12 cell lysate

    Predicted band size: 115 kDa

  • Flow Cytometry - Anti-BMPR2 antibody [1F12] (ab130206)
    Flow Cytometry - Anti-BMPR2 antibody [1F12] (ab130206)
    Overlay histogram showing HepG2 cells stained with ab130206 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130206, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR2 antibody [1F12] (ab130206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR2 antibody [1F12] (ab130206)
    ab130206 at 1/200 staining BMPR2 in paraffin-embedded tonsil tissue by immunohistochemistry with DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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