Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKN antibody [EPR3237] - BSA and Azide free (ab247729)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3237] to PKN - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PKN antibody [EPR3237] - BSA and Azide free
    See all PKN primary antibodies

  • Description

    Rabbit monoclonal [EPR3237] to PKN - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, SH-SY5Y, Jurkat and Human spleen lysates. IHC-P: Human brain tissue.

  • General notes

    ab247729 is the carrier-free version of ab108976.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PKN antibody [EPR3237] - BSA and Azide free (ab247729)
    Western blot - Anti-PKN antibody [EPR3237] - BSA and Azide free (ab247729)
    All lanes : Anti-PKN antibody [EPR3237] (ab108976) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : PKN1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 104 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab108976, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab108976 observed at 120 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab108976 Anti-PKN antibody [EPR3237] was shown to specifically react with PKN in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266599 (knockout cell lysate ab258586) was used. Wild-type and PKN knockout samples were subjected to SDS-PAGE. ab108976 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKN antibody [EPR3237] - BSA and Azide free (ab247729)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKN antibody [EPR3237] - BSA and Azide free (ab247729)
    This data was developed using ab108976, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of PKN in paraffin embedded Human brain tissue, using ab108976 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-PKN antibody [EPR3237] - BSA and Azide free (ab247729)
    Western blot - Anti-PKN antibody [EPR3237] - BSA and Azide free (ab247729)
    All lanes : Anti-PKN antibody [EPR3237] (ab108976) at 1/1000 dilution

    Lane 1 : Jurkat lysates
    Lane 2 : Human spleen lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 104 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    This data was developed using ab108976, the same antibody clone in a different buffer formulation.

  • Anti-PKN antibody [EPR3237] - BSA and Azide free (ab247729)
    Anti-PKN antibody [EPR3237] - BSA and Azide free (ab247729)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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