Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sbf1 antibody [EPR12567] (ab181106)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12567] to Sbf1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Sbf1 antibody [EPR12567]
    See all Sbf1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12567] to Sbf1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji, Human brain, Mouse cerebellum, and Mouse testis lysates. IHC-P: Human brain and muscle, and mouse cerebrum tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12567

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Sbf1 antibody [EPR12567] (ab181106)
    Western blot - Anti-Sbf1 antibody [EPR12567] (ab181106)
    All lanes : Anti-Sbf1 antibody [EPR12567] (ab181106) at 1/1000 dilution (Purified)

    Lane 1 : Mouse cerebellum lysates
    Lane 2 : Mouse testis lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 208 kDa
    Observed band size: 208 kDa



    We are unsure how to define the extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (ab181106)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (ab181106)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Sbf1 with purified ab181106 at 1/2000 dilution (0.54 µg/ml). Heat mediated antigen retrieval using Bond Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Sbf1 antibody [EPR12567] (ab181106)
    Western blot - Anti-Sbf1 antibody [EPR12567] (ab181106)
    All lanes : Anti-Sbf1 antibody [EPR12567] (ab181106) at 1/1000 dilution (Purified)

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
    Lane 2 : Human brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 208 kDa
    Observed band size: 208 kDa



    We are unsure how to define the extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (ab181106)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (ab181106)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Sbf1 with purified ab181106 at 1/2000 dilution (0.54 µg/ml). Heat mediated antigen retrieval using Bond Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (ab181106)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (ab181106)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Sbf1 with unpurified ab181106 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (ab181106)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (ab181106)

    Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling Sbf1 with unpurified ab181106 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-Sbf1 antibody [EPR12567] (ab181106)
    Anti-Sbf1 antibody [EPR12567] (ab181106)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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