Human ICA 512 / PTPRN ELISA Kit (ab226903)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.52 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture extracts, Cit plasma, Hep Plasma, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ICA 512 / PTPRN ELISA Kit
See all ICA 512/PTPRN kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell Extract 8 2.9% Inter-assay Sample n Mean SD CV% Cell Extract 3 6.6% -
Sample type
Cell culture extracts, Tissue, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.52 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 92 91% - 94% Tissue 87 85% - 90% Hep Plasma 85 82% - 87% Cit plasma 79 76% - 82% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human ICA 512 / PTPRN ELISA Kit (ab226903) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICA 512 / PTPRN protein in cit plasma, hep plasma, tissue, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ICA 512 / PTPRN with 3.52 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml 10X Human ICA 512 / PTPRN Capture Antibody 1 x 600µl 10X Human ICA 512 / PTPRN Detector Antibody 1 x 600µl Human ICA 512 / PTPRN Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Implicated in neuroendocrine secretory processes. May be involved in processes specific for neurosecretory granules, such as their biogenesis, trafficking or regulated exocytosis or may have a general role in neuroendocrine functions. Seems to lack intrinsic enzyme activity. May play a role in the regulation of secretory granules via its interaction with SNTB2. -
Tissue specificity
Expression is restricted to neuroendocrine cells. Found in pancreas, brain and pituitary. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 8 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
Post-translational
modificationsAppears to undergo multiple proteolytic cleavage at consecutive basic residues. -
Cellular localization
Membrane. Neuroendocrine secretory granules. - Information by UniProt
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Alternative names
- CA 512
- FLJ16131
- IA 2
see all -
Database links
- Entrez Gene: 5798 Human
- Omim: 601773 Human
- SwissProt: Q16849 Human
- Unigene: 89655 Human
Images
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Other - Human ICA 512 / PTPRN ELISA Kit (ab226903)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Human PTPRN standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of Human PTPRN standard curve in Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PTPRN in Human plasmasThe concentrations of PTPRN were measured in duplicates, interpolated from the PTPRN standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 25%, plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PTPRN concentration was determined to be 874.85 pg/mL in plasma (citrate) and 819.33 pg/mL in plasma (heparin).
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Interpolated concentrations of native PTPRN in Human SH-SY5Y cell extract and human pancreas extract based on a 500 µg/mL extract loadThe concentrations of PTPRN were measured in duplicate and interpolated from the PTPRN standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PTPRN concentration was determined to be 1478.61 pg/mL in SH-SY5Y extract and 489 pg/mL in human pancreas.
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Sandwich ELISA - Human ICA 512 / PTPRN ELISA Kit (ab226903)To learn more about the advantages of recombinant antibodies see here.
