Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸ 1 ml 459 004 ₸

Order now and get it on Thursday March 04, 2021

Anti-Wilms Tumor Protein antibody [SP321] (ab224806)

Key features and details

Rabbit monoclonal [SP321] to Wilms Tumor Protein
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human
Isotype: IgG

Product name

Anti-Wilms Tumor Protein antibody [SP321]
See all Wilms Tumor Protein primary antibodies
Description

Rabbit monoclonal [SP321] to Wilms Tumor Protein
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Wilms Tumor Protein (internal sequence).
Database link: P19544
Positive control
- IHC-P: Human ovarian carcinoma, Mouse testis, and Rat testis tissue; WB: K562 cell lysate; Flow Cyt: K-562 cells.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP321
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] (ab224806)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat testis tissue sections labeling Wilms Tumor Protein with ab224806 at 1/100 dilution (2.52 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] (ab224806)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Wilms Tumor Protein with ab224806 at 1/100 dilution (2.52 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] (ab224806)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling Wilms Tumor Protein with ab224806 at 1/100 dilution (2.52 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [SP321] (ab224806)

Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Wilms Tumor Protein using ab224806 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry - Anti-Wilms Tumor Protein antibody [SP321] (ab224806)

Flow Cytometry analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling Wilms Tumor Protein with purified ab224806 at 1:20 dilution (12.6 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
Western blot - Anti-Wilms Tumor Protein antibody [SP321] (ab224806)

Anti-Wilms Tumor Protein antibody [SP321] (ab224806) at 1/100 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Flow Cytometry - Anti-Wilms Tumor Protein antibody [SP321] (ab224806)

Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling Wilms Tumor Protein with ab224806 at 1/400 dilution (green) compared with a negative control of rabbit IgG (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com