Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] - BSA and Azide free (ab216646)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAN-R9(IHC)-56-2] to Wilms Tumor Protein - BSA and Azide free
- Suitable for: IHC-P, WB, ICC, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] - BSA and Azide free
See all Wilms Tumor Protein primary antibodies -
Description
Rabbit monoclonal [CAN-R9(IHC)-56-2] to Wilms Tumor Protein - BSA and Azide free -
Host species
Rabbit -
Specificity
Expression levels of the target protein vary with sample type and some optimisation may be required. -
Tested applications
Suitable for: IHC-P, WB, ICC, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562 and Ramos cell lysate. IHC-P: Human kidney and Wilms tumor tissues. ICC: K562 cells. Flow Cyt (intra): K562 cells.
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General notes
ab216646 is the carrier-free version of ab89901.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CAN-R9(IHC)-56-2 -
Isotype
IgG -
Research areas
Images
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Ab89901 staining Wilms Tumor Protein in paraffin-embedded Mouse testis tissue sections by Immunohistochemistry. Antigen retrieval was by heat mediation using citrate buffer (pH 6.0). Samples were incubated with primary antibody at 1:500 dilution (0.49 μg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Nuclear staining on Sertoli cells in mouse testis (PMID: 21863216).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab89901).
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Clone CAN-R9(IHC)-56-2 (ab216646) has been successfully conjugated by Abcam. This image was generated using Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (Alexa Fluor® 488). Please refer to ab202635 for protocol details.
ab202635 staining Wilms Tumor Protein in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202635 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5min).
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Flow cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Wilms Tumor Protein with ab89901 at 1/200 dilution (0.1μg)/ Red. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) / Black was used as the isotype control. Cells without incubation with primary antibody and secondary antibody / Blue was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab89901).
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Ab89901 staining Wilms Tumor Protein in paraffin-embedded Human ovarian serous adenocarcinoma tissue sections by Immunohistochemistry. Antigen retrieval was by heat mediation using citrate buffer (pH 6.0). Samples were incubated with primary antibody at 1:500 dilution (0.49 μg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Nuclear staining on human ovarian serous adenocarcinoma (PMID: 11939727).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab89901).
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Ab89901 staining Wilms Tumor Protein in paraffin-embedded Human kidney tissue sections by Immunohistochemistry. Antigen retrieval was by heat mediation using citrate buffer (pH 6.0). Samples were incubated with primary antibody at 1:500 dilution (0.49 μg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Nuclear staining on human kidney glomerulus (PMID: 12898605).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab89901).
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Immunohistochemical analysis of fromaldehyde fixed, paraffin embedded rat testis tissue sections, labelling Wilms Tumor Protein using ab89901. Heat mediated antigen retrival was performed using 10 mM Sodium Citrate and 0.05% Tween 20. Tissue sections were incubated with ab89901 at a 1/50 dilution for 12 hours at 4ºC. The tissues were blocked with 10% Serum for 30 minutes at 25ºC. The secondary used was a Donkey CY3® conjugate at a 1/200 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab89901).
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Clone CAN-R9(IHC)-56-2 (ab216646) has been successfully conjugated by Abcam. This image was generated using Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (Alexa Fluor® 647). Please refer to ab202639 for protocol details.
ab202639 staining Wilms Tumor Protein in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202639 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Immunocytochemsitry/Immunofluorescence analysis of K562 cells labelling Wilms Tumor Protein (green) with purified ab89901 at 1/50. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab89901).
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Immunocytochemsitry/Immunofluorescence analysis of K562 cells labelling Wilms Tumor Protein (green) with unpurified ab89901 at 1/50. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab89901).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal tissue labelling Wilms Tumor Protein with unpurified ab89901 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab89901).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Wilms tumor tissue labelling Wilms Tumor Protein with unpurified ab89901 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab89901).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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