IHC image of Wilms Tumor Protein staining in a section of formalin-fixed paraffin-embedded Human Wilms Tumor performed on a Ventana Discovery Ultra system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20mins. The section was then incubated with ab15249, 1/100, for 16 mins at 37°C and detected using an HRP conjugated anti-rabbit secondary antibody. DAB was used as the chromogen and the section was then counterstained with haematoxylin.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC image of Wilms Tumor Protein staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BOND^TM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15249, 1/100, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre