Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3206(2)] to Galectin 1
Suitable for: Flow Cyt, WB, IHC-P
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-Galectin 1 antibody [EPR3206(2)]
See all Galectin 1 primary antibodies
Description

Rabbit monoclonal [EPR3206(2)] to Galectin 1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Galectin 1 aa 50-150. The exact sequence is proprietary.
Positive control
- HEK293T, K562, Human placenta and fetal kidney lysates, Human kidney and Human skin tissues
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3206(2)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Galectin 1 with ab138513 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

All lanes : Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : LGALS1 knockout HEK-293T cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Lanes 1- 2: Merged signal (red and green). Green - ab138513 observed at 15 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab138513 was shown to react with Galectin 1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266850 (knockout cell lysate ab256974) was used. Wild-type HEK-293T and LGALS1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab138513 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

All lanes : Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : LGALS1 (Galectin 1) knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 15 kDa

Lanes 1 - 2: Merged signal (red and green). Green - ab138513 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab138513 was shown to specifically react with Galectin 1 in wild-type HAP1 cells as signal was lost in LGALS1 (Galectin 1) knockout cells. Wild-type and LGALS1 (Galectin 1) knockout samples were subjected to SDS-PAGE. ab138513 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

All lanes : Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/1000 dilution

Lane 1 : K562 lysate
Lane 2 : Human placenta lysate
Lane 3 : Fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 15 kDa
Observed band size: 14 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

Flow cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) labeling Galectin 1 with ab138513 at 1/100 dilution (red). ab150077 at 1/2000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde
and permeabilised with 90% methanol.

Isotype control - Rabbit monoclonal IgG, ab172730 (Black)
Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Galectin 1 with ab138513 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

Immunohistochemical analysis of paraffin embedded Normal Human Stomach tissue using ab138513 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

Immunohistochemical analysis of paraffin embedded Normal Human Spleen tissue using ab138513 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

Immunohistochemical analysis of paraffin embedded Normal Human Placenta tissue using ab138513 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

Immunohistochemical analysis of paraffin embedded Normal Human Heart tissue using ab138513 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab138513 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

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