Anti-Galectin 1 antibody (ab38328)
Key features and details
- Rabbit polyclonal to Galectin 1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Galectin 1 antibody
See all Galectin 1 primary antibodies -
Description
Rabbit polyclonal to Galectin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Galectin 1 aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab38327) -
Positive control
- Recombinant human Galectin 1 protein (ab50237) can be used as a positive control in WB. ab38328 gave a positive signal in the following Human Lysates: Heart, Skeletal Muscle, Kidney (Data not shown), Thymus (Data not shown), HeLa, A431, HL60 (Data not shown), Ramos (Data not shown) and THP1 (Data not shown).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatSheepCowDogPigChinese hamsterOrangutanApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).Target
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Function
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. -
Tissue specificity
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 326598 Cow
- Entrez Gene: 3956 Human
- Entrez Gene: 16852 Mouse
- Entrez Gene: 414915 Pig
- Entrez Gene: 56646 Rat
- Omim: 150570 Human
- SwissProt: P11116 Cow
- SwissProt: P09382 Human
see all -
Alternative names
- 14 kDa laminin-binding protein antibody
- 14 kDa lectin antibody
- Beta galactoside binding lectin antibody
see all
Images
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Western blot - Anti-Galectin 1 antibody (ab38328)All lanes : Anti-Galectin 1 antibody (ab38328) at 1 µg
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : LGALS1 (Galectin 1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 14 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab38328 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab38328 was shown to recognize Galectin 1 in wild-type HAP1 cells as signal was lost at the expected MW in LGALS1 (Galectin 1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LGALS1 (Galectin 1) knockout samples were subjected to SDS-PAGE. Ab38328 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Galectin 1 antibody (ab38328)All lanes : Anti-Galectin 1 antibody (ab38328) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 34 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab38328 has been referenced in 2 publications.
- Stegemann C et al. Proteomic identification of matrix metalloproteinase substrates in the human vasculature. Circ Cardiovasc Genet 6:106-17 (2013). WB . PubMed: 23255316
- Woolley JF & Al-Rubeai M The isolation and identification of a secreted biomarker associated with cell stress in serum-free CHO cell culture. Biotechnol Bioeng 104:590-600 (2009). WB ; Chinese hamster . PubMed: 19544303
Images
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Western blot - Anti-Galectin 1 antibody (ab38328)All lanes : Anti-Galectin 1 antibody (ab38328) at 1 µg
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : LGALS1 (Galectin 1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 14 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab38328 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab38328 was shown to recognize Galectin 1 in wild-type HAP1 cells as signal was lost at the expected MW in LGALS1 (Galectin 1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LGALS1 (Galectin 1) knockout samples were subjected to SDS-PAGE. Ab38328 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Galectin 1 antibody (ab38328)All lanes : Anti-Galectin 1 antibody (ab38328) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 34 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
