Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VEGF Receptor 2 antibody [EPR21884-290] - BSA and Azide free (ab234101)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21884-290] to VEGF Receptor 2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-VEGF Receptor 2 antibody [EPR21884-290] - BSA and Azide free
    See all VEGF Receptor 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR21884-290] to VEGF Receptor 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat placenta and lung lysates; bEnd.3 whole cell lysate.

  • General notes

    Ab234101 is the carrier-free version of ab221679. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab234101 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-VEGF Receptor 2 antibody [EPR21884-290] - BSA and Azide free (ab234101)
    Western blot - Anti-VEGF Receptor 2 antibody [EPR21884-290] - BSA and Azide free (ab234101)
    All lanes : Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679) at 1/1000 dilution

    Lane 1 : Mouse placenta lysate at 20 µg
    Lane 2 : Rat placenta lysate at 20 µg
    Lane 3 : Rat lung lysate at 20 µg
    Lane 4 : Mouse lung lysate at 20 µg
    Lane 5 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate at 10 µg

    Secondary
    Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
    Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 152 kDa
    Observed band size: 180-230 kDa
    why is the actual band size different from the predicted?



    Exposure time :  Lanes 1-2: 3 minutes; Lane 3: 37 seconds; Lane 4: 59 seconds; Lane 5: 15 seconds

    The molecular mass observed is consistent with the literature (PMID: 23354168 ; PMID: 20606037).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221679).

  • Anti-VEGF Receptor 2 antibody [EPR21884-290] - BSA and Azide free (ab234101)
    Anti-VEGF Receptor 2 antibody [EPR21884-290] - BSA and Azide free (ab234101)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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