Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22395-52] to DC-SIGN - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free
    See all DC-SIGN primary antibodies

  • Description

    Rabbit monoclonal [EPR22395-52] to DC-SIGN - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: THP-1 cells. Flow: THP-1 cells.

  • General notes

    Ab245200 is the carrier-free version of ab245189. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab245200 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)
    Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) cells labeling DC-SIGN using ab245189 at 1/100 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in THP-1 cells treated with 10 ng/ml PMA for 18h, then serum starved for 8 hours, then 10 ng/ml PMA for 6 hours and add 1000 U IL4 for 2 hours, then add 10% serum for another 22 hours. DC-SIGN expression is induced by PMA plus IL4 in THP-1 (PMID: 15070901; PMID: 22675249). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to stain tubulin (red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245189).

  • Flow Cytometry - Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)
    Flow Cytometry - Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)

    Flow cytometric analysis of 4% paraformaldehyde-fixed THP-1 (human monocytic leukemia cell line) cells treated with 10 ng/ml PMA for 18 hours, then serum starved for 8 hours, then 10 ng/ml PMA for 6 hours and add 1000 U IL4 for 2 hours, then add 10% serum for another 22 hours. DC-SIGN is labeled with ab245189 at 1/60 dilution in treated (red) and untreated cells (green) as compared to a Rabbit monoclonal IgG (ab172730, black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody, blue).  A Goat anti rabbit IgG (Dylight ® 488, ab98462) was used as the secondary antibody at 1/2000 dilution. Gated on viable cells. DC-SIGN expression is induced by PMA plus IL4 in THP-1 (PMID: 15070901; PMID: 22675249).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245189).

  • Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)
    Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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