Recombinant P. falciparum PfHDAC1 protein (Active) (ab271711)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 58% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant P. falciparum PfHDAC1 protein (Active) -
Biological activity
Specific Activity: ≥1.85 pmol/min/µg.Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3, and pf-HDAC1. HDAC reaction was performed for 30 mins at 37°C followed by treatment with 50 µl of HDAC developer for 15 mins at RT. Fluorescence intensity is measured at ex360/em460. -
Purity
>= 58 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Plasmodium falciparum -
Sequence
MSNRKKVAYF HDPDIGSYYY GAGHPMKPQR IRMTHSLIVS YNLYKYMEVY RPHKSDVNEL TLFHDYEYID FLSSISLENY REFTYQLKRF NVGEATDCPV FDGLFQFQQS CAGASIDGAS KLNHHCADIC VNWSGGLHHA KMSEASGFCY INDIVLGILE LLKYHARVMY IDIDVHHGDG VEEAFYVTHR VMTVSFHKFG DYFPGTGDIT DVGVNHGKYY SVNVPLNDGM TDDAFVDLFK VVIDKCVQTY RPGAIIIQCG ADSLTGDRLG RFNLTIKGHA RCVEHVRSYN IPLLVLGGGG YTIRNVSRCW AYETGVVLNK HHEMPDQISL NDYYDYYAPD FQLHLQPSNI PNYNSPEHLS RIKMKIAENL RHIEHAPGVQ FSYVPPDFFN SDIDDESDKN QYELKDDSGG GRAPGTRAKE HSTTHHLRRK NYDDDFFDLS DRDQSIVPY -
Predicted molecular weight
53 kDa including tags -
Amino acids
1 to 449 -
Tags
His tag C-Terminus , DDDDK tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol (glycerin, glycerine)
90 µg/ml FLAG peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant P. falciparum PfHDAC1 protein (Active) (ab271711)SDS-PAGE analysis of ab271711.
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Functional Studies - Recombinant P. falciparum PfHDAC1 protein (Active) (ab271711)Specific activity of ab271711 ≥1.85 pmol/min/µg.