Anti-BIG1 antibody (ab72061)
Key features and details
- Rabbit polyclonal to BIG1
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BIG1 antibody
See all BIG1 primary antibodies -
Description
Rabbit polyclonal to BIG1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to a region between residue 1800 and the C-terminus (residue 1849) of human BIG1 (according to NP_006412.2)
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Positive control
- Whole cell lysate from HeLa, 293T or NIH3T3 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody (ab72061)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BIG1 with ab72061 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-BIG1 antibody (ab72061)All lanes : Anti-BIG1 antibody (ab72061) at 0.1 µg/ml
Lane 1 : Whole
cell lysate from HeLa cells at 50 µg
Lane 2 : Whole
cell lysate from HeLa cells at 15 µg
Lane 3 : Whole
cell lysate from HeLa cells at 5 µg
Lane 4 : Whole
cell lysate from 293T cells at 50 µg
Lane 5 : Whole
cell lysate from NIH 3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 208 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa, 41 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Immunoprecipitation - Anti-BIG1 antibody (ab72061)Detection of Human BIG1 by Immunoprecipitation in 1mg whole cell lysate from HeLa cells, using ab72061 at 3 µg/mg lysate (lane 1) or a control rabbit IgG (lane 2). Subsequent Western blot detection was performed using ab72061 at 1 µg/ml on 20% of the imunoprecipitate.
