Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRADD antibody [EPR3604] (ab110644)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3604] to TRADD
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TRADD antibody [EPR3604]
    See all TRADD primary antibodies

  • Description

    Rabbit monoclonal [EPR3604] to TRADD

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, MCF7, HeLa and K562 cell lysates, Human liver, and Human fetal spleen lysates. IHC-P: human breast carcinoma tissue. ICC/IF: MCF7 and HeLa cells. Flow Cyt: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-TRADD antibody [EPR3604] (ab110644)
    Western blot - Anti-TRADD antibody [EPR3604] (ab110644)
    All lanes : Anti-TRADD antibody [EPR3604] (ab110644) at 1/1000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 2 : Human liver lysates
    Lane 3 : Humanf fetal spleen lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody [EPR3604] (ab110644)
    Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody [EPR3604] (ab110644)
    Immunocytochemistry/ Immunofluorescence analysis of MCF7(Human breast adenocarcinoma epithelial cell) cells labeling TRADD with purified ab110644 at 1/100 dilution (6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] (ab110644)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] (ab110644)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling TRADD with purified ab110644 at 1/850 dilution (0.7 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry - Anti-TRADD antibody [EPR3604] (ab110644)
    Flow Cytometry - Anti-TRADD antibody [EPR3604] (ab110644)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TRADD with purified ab110644 at 1/600 dilution (1 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] (ab110644)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] (ab110644)

    Unpurified ab110644, at 1/100, staining TRADD in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-TRADD antibody [EPR3604] (ab110644)
    Western blot - Anti-TRADD antibody [EPR3604] (ab110644)
    All lanes : Anti-TRADD antibody [EPR3604] (ab110644) at 1/500 dilution ((unpurified))

    Lane 1 : Jurkat cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody [EPR3604] (ab110644)
    Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody [EPR3604] (ab110644)

    Unpurified ab110644, at 1/100, staining TRADD in HeLa cells by Immunofluorescence.

  • Anti-TRADD antibody [EPR3604] (ab110644)
    Anti-TRADD antibody [EPR3604] (ab110644)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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