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Anti-TRADD antibody (ab223040)

Anti-TRADD antibody (ab223040)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to TRADD
  • Suitable for: IP, WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TRADD antibody
    See all TRADD primary antibodies
  • Description

    Rabbit polyclonal to TRADD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human TRADD aa 1-312.
    Database link: Q15628

  • Positive control

    • WB: K562 cell lysate; Rat kidney tissue lysate. IHC-P: Human liver and tonsil tissues. ICC/IF: U251 cells. IP: K562 cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Extracellular Signals
    • Death Ligands
    • Cell Biology
    • Apoptosis
    • Receptors
    • Death Receptors
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • TRADD
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • TRADD

Images

  • Western blot - Anti-TRADD antibody (ab223040)
    Western blot - Anti-TRADD antibody (ab223040)
    All lanes : Anti-TRADD antibody (ab223040) at 1/500 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : Rat kidney tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 28,35 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody (ab223040)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody (ab223040)

    Paraffin-embedded human liver tissue stained for TRADD using ab223040 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody (ab223040)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody (ab223040)

    Paraffin-embedded human tonsil tissue stained for TRADD using ab223040 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody (ab223040)
    Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody (ab223040)

    U251 cells stained for TRADD (green) using ab223040 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunoprecipitation - Anti-TRADD antibody (ab223040)
    Immunoprecipitation - Anti-TRADD antibody (ab223040)

    TRADD was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate with ab223040 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab223040 at 1/500 dilution. HRP-conjugated Protein G antibody was used as secondary antibody at 1/2000 dilution.

    Lane 1: Rabbit control IgG instead of ab223040 in K562 whole cell lysate.

    Lane 2: ab223040 IP in K562 whole cell lysate. 

    Lane 3: K562 whole cell lysate 10 µg (Input). 

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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