Anti-TRADD antibody (ab223040)
Key features and details
- Rabbit polyclonal to TRADD
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TRADD antibody
See all TRADD primary antibodies -
Description
Rabbit polyclonal to TRADD -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human TRADD aa 1-312.
Database link: Q15628 -
Positive control
- WB: K562 cell lysate; Rat kidney tissue lysate. IHC-P: Human liver and tonsil tissues. ICC/IF: U251 cells. IP: K562 cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-TRADD antibody (ab223040) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : Rat kidney tissue lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 28,35 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
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Paraffin-embedded human liver tissue stained for TRADD using ab223040 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human tonsil tissue stained for TRADD using ab223040 at 1/100 dilution in immunohistochemical analysis.
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U251 cells stained for TRADD (green) using ab223040 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
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TRADD was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate with ab223040 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab223040 at 1/500 dilution. HRP-conjugated Protein G antibody was used as secondary antibody at 1/2000 dilution.
Lane 1: Rabbit control IgG instead of ab223040 in K562 whole cell lysate.
Lane 2: ab223040 IP in K562 whole cell lysate.
Lane 3: K562 whole cell lysate 10 µg (Input).