Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [2C10A12] to ATG3
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-ATG3 antibody [2C10A12]
See all ATG3 primary antibodies
Description

Mouse monoclonal [2C10A12] to ATG3
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human ATG3 aa 1-100. Expressed in E. coli.
Database link: Q9NT62

Run BLAST with Run BLAST with
Positive control
- WB: Recombinant human ATG3 protein (amino acids 1-100). ATG3 (amino acids: 1-100) - hIgGFc transfected HEK-293 cell lysate. HeLa, K562 and THP-1 cell lysate. ICC/IF: SMMC-7721 cells. Flow Cytometry: Jurkat cells. IHC-P: Human stomach cancer and rectum cancer tissue.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

2C10A12
Isotype

IgG1
Research areas

Western blot - Anti-ATG3 antibody [2C10A12] (ab233562)

All lanes : Anti-ATG3 antibody [2C10A12] (ab233562) at 1/500 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : THP-1 (Human monocytic leukemia cell line) cell lysate

Predicted band size: 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ATG3 antibody [2C10A12] (ab233562)

SMMC-7721 cells stained for ATG3 (green) using ab233562 (1/200 dilution) in ICC/IF.

Blue: DRAQ5 fluorescent DNA dye.

Red: Actin filaments have been labeled with Alexa Fluor^®555 phalloidin.

Secondary antibody: Goat anti-Mouse IgG (H+L) DyLight 488.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG3 antibody [2C10A12] (ab233562)

Paraffin-embedded human rectum cancer tissue stained for ATG3 with ab233562 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
Flow Cytometry - Anti-ATG3 antibody [2C10A12] (ab233562)

Flow Cytometry analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells, labeling ATG3 with ab233562 at a 1/200 dilution (green) compared to a negative control (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG3 antibody [2C10A12] (ab233562)

Paraffin-embedded human stomach cancer tissue stained for ATG3 with ab233562 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
Western blot - Anti-ATG3 antibody [2C10A12] (ab233562)

All lanes : Anti-ATG3 antibody [2C10A12] (ab233562) at 1/500 dilution

Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Apg3 (amino acids: 1-100) - hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 36 kDa
Western blot - Anti-ATG3 antibody [2C10A12] (ab233562)

Anti-ATG3 antibody [2C10A12] (ab233562) at 1/500 dilution + Recombinant human Apg3 protein (amino acids 1-100)

Predicted band size: 36 kDa

(Expected MW is 37.3 kDa)

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