Anti-ATG3 antibody [2C10A12] (ab233562)
![Anti-ATG3 antibody [2C10A12] (ab233562)](https://www.abcam.com/ps/products/233/ab233562/Images/ab233562-306743-anti-atg3-antibody-2c10a12-western-blot.jpg "Anti-ATG3 antibody [2C10A12] (ab233562)")
Key features and details
- Mouse monoclonal [2C10A12] to ATG3
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ATG3 antibody [2C10A12]
See all ATG3 primary antibodies -
Description
Mouse monoclonal [2C10A12] to ATG3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human ATG3 aa 1-100. Expressed in E. coli.
Database link: Q9NT62 -
Positive control
- WB: Recombinant human ATG3 protein (amino acids 1-100). ATG3 (amino acids: 1-100) - hIgGFc transfected HEK-293 cell lysate. HeLa, K562 and THP-1 cell lysate. ICC/IF: SMMC-7721 cells. Flow Cytometry: Jurkat cells. IHC-P: Human stomach cancer and rectum cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
2C10A12 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-ATG3 antibody [2C10A12] (ab233562)All lanes : Anti-ATG3 antibody [2C10A12] (ab233562) at 1/500 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : THP-1 (Human monocytic leukemia cell line) cell lysate
Predicted band size: 36 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-ATG3 antibody [2C10A12] (ab233562)](https://www.abcam.com/ps/products/233/ab233562/Images/ab233562-306744-anti-atg3-antibody-2c10a12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATG3 antibody [2C10A12] (ab233562)SMMC-7721 cells stained for ATG3 (green) using ab233562 (1/200 dilution) in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye.
Red: Actin filaments have been labeled with Alexa Fluor®555 phalloidin.
Secondary antibody: Goat anti-Mouse IgG (H+L) DyLight 488.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG3 antibody [2C10A12] (ab233562)](https://www.abcam.com/ps/products/233/ab233562/Images/ab233562-306747-anti-atg3-antibody-2c10a12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG3 antibody [2C10A12] (ab233562)Paraffin-embedded human rectum cancer tissue stained for ATG3 with ab233562 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
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![Flow Cytometry - Anti-ATG3 antibody [2C10A12] (ab233562)](https://www.abcam.com/ps/products/233/ab233562/Images/ab233562-306745-anti-atg3-antibody-2c10a12-flow-cytometry.jpg)
Flow Cytometry - Anti-ATG3 antibody [2C10A12] (ab233562)Flow Cytometry analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells, labeling ATG3 with ab233562 at a 1/200 dilution (green) compared to a negative control (red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG3 antibody [2C10A12] (ab233562)](https://www.abcam.com/ps/products/233/ab233562/Images/ab233562-306746-anti-atg3-antibody-2c10a12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG3 antibody [2C10A12] (ab233562)Paraffin-embedded human stomach cancer tissue stained for ATG3 with ab233562 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
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![Western blot - Anti-ATG3 antibody [2C10A12] (ab233562)](https://www.abcam.com/ps/products/233/ab233562/Images/ab233562-306742-anti-atg3-antibody-2c10a12-western-blot.jpg)
Western blot - Anti-ATG3 antibody [2C10A12] (ab233562)All lanes : Anti-ATG3 antibody [2C10A12] (ab233562) at 1/500 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Apg3 (amino acids: 1-100) - hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 36 kDa
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![Western blot - Anti-ATG3 antibody [2C10A12] (ab233562)](https://www.abcam.com/ps/products/233/ab233562/Images/ab233562-306741-anti-atg3-antibody-2c10a12-western-blot.jpg)
Western blot - Anti-ATG3 antibody [2C10A12] (ab233562)Anti-ATG3 antibody [2C10A12] (ab233562) at 1/500 dilution + Recombinant human Apg3 protein (amino acids 1-100)
Predicted band size: 36 kDa(Expected MW is 37.3 kDa)
