Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [7A1D1] to ATG3
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human, African green monkey
Isotype: IgG1

Product name

Anti-ATG3 antibody [7A1D1]
See all ATG3 primary antibodies
Description

Mouse monoclonal [7A1D1] to ATG3
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human ATG3 aa 1-100. Expressed in E. coli.
Database link: Q9NT62

Run BLAST with Run BLAST with
Positive control
- WB: Recombinant human ATG3 protein (amino acids 1-100). ATG3 (AA: 1-100) - hIgGFc transfected HEK-293 cell lysate. HeLa, COS-7, Jurkat, THP-1 and K562 cell lysate. ICC/IF: HeLa and SMMC-7721 cells. IHC-P: Human breast cancer and esophageal cancer tissue.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

7A1D1
Isotype

IgG1
Research areas

Western blot - Anti-ATG3 antibody [7A1D1] (ab233560)

All lanes : Anti-ATG3 antibody [7A1D1] (ab233560) at 1/500 dilution

Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : THP-1 (Human monocytic leukemia cell line) cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

Predicted band size: 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ATG3 antibody [7A1D1] (ab233560)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for ATG3 (green) using ab233560 (1/200 dilution) in ICC/IF.

Blue: DRAQ5 fluorescent DNA dye.

Red: Actin filaments have been labeled with Alexa Fluor^®555 phalloidin.

Secondary antibody: Goat anti-Mouse IgG (H+L) DyLight 488.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG3 antibody [7A1D1] (ab233560)

Paraffin-embedded human esophageal cancer tissue stained for ATG3 with ab233560 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG3 antibody [7A1D1] (ab233560)

Paraffin-embedded human breast cancer tissue stained for ATG3 with ab233560 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
Immunocytochemistry/ Immunofluorescence - Anti-ATG3 antibody [7A1D1] (ab233560)

SMMC-7721 cells stained for ATG3 (green) using ab233560 (1/200 dilution) in ICC/IF.

Blue: DRAQ5 fluorescent DNA dye.

Red: Actin filaments have been labeled with Alexa Fluor^®555 phalloidin.

Secondary antibody: Goat anti-Mouse IgG (H+L) DyLight 488.
Western blot - Anti-ATG3 antibody [7A1D1] (ab233560)

All lanes : Anti-ATG3 antibody [7A1D1] (ab233560) at 1/500 dilution

Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : ATG3 (amino acids 1-100) - hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 36 kDa
Western blot - Anti-ATG3 antibody [7A1D1] (ab233560)

Anti-ATG3 antibody [7A1D1] (ab233560) at 1/500 dilution + Recombinant human ATG3 protein (amino acids 1-100)

Predicted band size: 36 kDa

(Expected MW is 37.3 kDa)

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