Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4802] to ATG3
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-ATG3 antibody [EPR4802]
See all ATG3 primary antibodies
Description

Rabbit monoclonal [EPR4802] to ATG3
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK293T, K562, HeLa or Jurkat cell lysates. Flow Cyt: Permeabilized Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4802
Isotype

IgG
Research areas

Western blot - Anti-ATG3 antibody [EPR4802] (ab108282)

All lanes : Anti-ATG3 antibody [EPR4802] (ab108282) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ATG3 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 36 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab108282 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.

ab108282 Anti-ATG3 antibody [EPR4802] was shown to specifically react with ATG3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266707 (knockout cell lysate ab257363) was used. Wild-type and ATG3 knockout samples were subjected to SDS-PAGE. ab108282 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-ATG3 antibody [EPR4802] (ab108282)

Flow cytometric analysis of permeabilized Jurkat cells using ab108282 at 1/10 (red) or a rabbit IgG (negative) (green).
Western blot - Anti-ATG3 antibody [EPR4802] (ab108282)

All lanes : Anti-ATG3 antibody [EPR4802] (ab108282) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Anti-ATG3 antibody [EPR4802] (ab108282)

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