Anti-ATG3 antibody (ab223522)
Key features and details
- Rabbit polyclonal to ATG3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ATG3 antibody
See all ATG3 primary antibodies -
Description
Rabbit polyclonal to ATG3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human ATG3 (internal sequence).
Database link: Q9NT62 -
Positive control
- WB: HeLa whole cell lysate. ICC/IF: SK-N-BE cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-ATG3 antibody (ab223522)
4% formaldehyde-fixed SK-N-BE cells stained for ATG3 (green) using ab223522 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min at RT, 5 min at RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223522 (D) Merge.
-
Western blot - Anti-ATG3 antibody (ab223522)Anti-ATG3 antibody (ab223522) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 35,36 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutesBlocking buffer: 5% skim milk in TBST.
Primary and secondary antibodies were incubated for 1 hour at RT.
