Anti-ATG3 antibody (ab228749)
Key features and details
- Rabbit polyclonal to ATG3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ATG3 antibody
See all ATG3 primary antibodies -
Description
Rabbit polyclonal to ATG3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant fragment within Human ATG3 (internal sequence). The exact sequence is proprietary.
Database link: Q9NT62 -
Positive control
- WB: HeLa whole cell extract; Jurkat, Raji, PC-12, Rat2, C8D30, NIH/3T3, BCL-1, RAW 264.7, K 562, THP-1 and HL-60 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATG3 antibody (ab228749)All lanes : Anti-ATG3 antibody (ab228749) at 1/1000 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : Apg3 shRNA-transfected HeLa whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa10% SDS-PAGE gel.
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Western blot - Anti-ATG3 antibody (ab228749)All lanes : Anti-ATG3 antibody (ab228749) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa10% SDS-PAGE gel.
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Western blot - Anti-ATG3 antibody (ab228749)All lanes : Anti-ATG3 antibody (ab228749) at 1/2000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa10% SDS-PAGE gel.
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Western blot - Anti-ATG3 antibody (ab228749)All lanes : Anti-ATG3 antibody (ab228749) at 1/1000 dilution
Lane 1 : C8D30 whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa10% SDS-PAGE gel.
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Western blot - Anti-ATG3 antibody (ab228749)All lanes : Anti-ATG3 antibody (ab228749) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa10% SDS-PAGE gel.
