Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)
Key features and details
- Rabbit polyclonal to eEF1A1+eEF1A2+eEF1AL3
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-eEF1A1+eEF1A2+eEF1AL3 antibody -
Description
Rabbit polyclonal to eEF1A1+eEF1A2+eEF1AL3 -
Host species
Rabbit -
Specificity
Due to sequence homology the antibody recognises all three eEF1A forms, eEF1A1, eEF1A2 and eEF1A3. Through WB ab37969 was found to detect recombinant and endogenous eF1A1 and eF1A2. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Hamster, Cow, Cat, Dog, Pig, Chimpanzee, Macaque monkey
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Immunogen
Synthetic peptide corresponding to Human eEF1A1+eEF1A2+eEF1AL3 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab37968) -
Positive control
- This antibody gave a positive signal in the following: Human Whole Cell Lysates (HeLa, Jurkat, A431, HEK 293, HepG2, MCF-7, SHSY-5Y, U2OS) Mouse Whole Cell Lysates (NIH 3T3, MEF1) Mouse Tissue Lysates (Brain, Liver, Heart, Kidney, Pancreas, Testis, Skeletal muscle, Spinal Cord, Ovary) Rat Whole Cell Lysate(PC12) Rat Tissue Lysate (Brain, Liver, Heart)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab37969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF (1) Use a concentration of 1 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Notes ICC/IF
Use a concentration of 1 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Target
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Relevance
Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Tissue specificity eEF1A1: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle. Tissue specificity eEF1A2: Brain, heart, and skeletal muscle. Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. PTM eEF1A1: ISGylated. -
Database links
- SwissProt: P68104 Human
- SwissProt: Q05639 Human
- SwissProt: Q5VTE0 Human
- SwissProt: P10126 Mouse
- SwissProt: P62630 Rat
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Alternative names
- CCS3 antibody
- eEF1A-1 antibody
- eEF1A-2 antibody
see all
Images
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Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lane 4 :HEK-293 whole cell lysate (ab7902)
Lane 5 : Hep G2 whole cell lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)ICC/IF image of ab37969 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37969, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). -
Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : Heart (Mouse) Tissue Lysate
Lane 6 : Kidney (Mouse) Tissue Lysate
Lane 7 :Mouse pancreas tissue lysate - total protein (ab29363)
Lane 8 : Testis (Mouse) Tissue Lysate
Lane 9 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 10 : Spinal Cord (Mouse) Tissue Lysate
Lane 11 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 12 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 13 : Brain (Rat) Tissue Lysate - normal tissue
Lane 14 : Liver (Rat) Tissue Lysate
Lane 15 : Heart (Rat) Tissue Lysate
Lane 16 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 :Recombinant Human eEF1A1/EF-Tu protein (ab81792)
Lane 2 : Human EEF1A2 full length protein (ab40317)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab37969 has been referenced in 10 publications.
- Endicott SJ et al. Long-lived mice with reduced growth hormone signaling have a constitutive upregulation of hepatic chaperone-mediated autophagy. Autophagy 17:612-625 (2021). PubMed: 32013718
- Wu M et al. URGCP/URG4 promotes apoptotic resistance in bladder cancer cells by activating NF-?B signaling. Oncotarget 6:30887-901 (2015). PubMed: 26429874
- Dong C et al. Proteasome modulates positive and negative translational regulators in long-term synaptic plasticity. J Neurosci 34:3171-82 (2014). PubMed: 24573276
- Häsler J et al. Cytoplasmic activation-induced cytidine deaminase (AID) exists in stoichiometric complex with translation elongation factor 1a (eEF1A). Proc Natl Acad Sci U S A 108:18366-71 (2011). PubMed: 22042842
- Hashimoto K & Ishima T Neurite Outgrowth Mediated by Translation Elongation Factor eEF1A1: A Target for Antiplatelet Agent Cilostazol. PLoS One 6:e17431 (2011). WB ; Rat . PubMed: 21390260
- Vessey JP et al. Mammalian Pumilio 2 regulates dendrite morphogenesis and synaptic function. Proc Natl Acad Sci U S A 107:3222-7 (2010). WB . PubMed: 20133610
- Lomenick B et al. Target identification using drug affinity responsive target stability (DARTS). Proc Natl Acad Sci U S A 106:21984-9 (2009). WB ; Human . PubMed: 19995983
- Taylor AM et al. Axonal mRNA in uninjured and regenerating cortical mammalian axons. J Neurosci 29:4697-707 (2009). ICC/IF ; Rat . PubMed: 19369540
- Andersen KM et al. Thioredoxin Txnl1/TRP32 is a redox-active cofactor of the 26 S proteasome. J Biol Chem 284:15246-54 (2009). WB ; Human . PubMed: 19349277
- Eleuterio E et al. Proteome analysis of X-ray irradiated human erythroleukemia cells. Biochim Biophys Acta 1784:611-20 (2008). PubMed: 18302948
Images
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Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lane 4 :HEK-293 whole cell lysate (ab7902)
Lane 5 : Hep G2 whole cell lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)ICC/IF image of ab37969 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37969, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : Heart (Mouse) Tissue Lysate
Lane 6 : Kidney (Mouse) Tissue Lysate
Lane 7 :Mouse pancreas tissue lysate - total protein (ab29363)
Lane 8 : Testis (Mouse) Tissue Lysate
Lane 9 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 10 : Spinal Cord (Mouse) Tissue Lysate
Lane 11 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 12 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 13 : Brain (Rat) Tissue Lysate - normal tissue
Lane 14 : Liver (Rat) Tissue Lysate
Lane 15 : Heart (Rat) Tissue Lysate
Lane 16 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 :Recombinant Human eEF1A1/EF-Tu protein (ab81792)
Lane 2 : Human EEF1A2 full length protein (ab40317)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Exposure time: 3 minutes